The good, bad and ugly: VITROS Biotin story

Satish Ramanathan, Srinivas Chakravarthy

Abstract

Biotin is a potential interferent with immunoassays and competetitve assays being positively interfered and sandwich assays being negatively interfered. Immunoassay platforms utilising streptavidin biotin complexes are bound to such interferences. Hence, selection and verification of immunoassay method has become an essential requirement for the users. Our case scenario discusses the influence of biotin on HIV assay in two platforms, one utilising the streptavidin biotin design and the other without it.

Keywords

biotin; immunoassay; false positive; HIV

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References

Ortho Clinical Diagnostics; Pub. No. GEM 1250_XUS_EN Version 10.1

Beauty fad’s ugly downside: test interference; Viewed September 2016, www.captodayonline.com/beauty-fads-ugly-Downside test-interference/

Put in on the board; 11/17; Viewed November 2017, www.captodayonline.com/put-board-1117/

Immunoassay Interference; Viewed November 2016, captoday.epubxp.com/i/751425-nov-2016/12


DOI: http://dx.doi.org/10.18063/apm.v2i2.556
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