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Plasma Mutation Breeding of High Yield γ-Aminobutyric Acid Lactic Acid Bacteria

Gao Chengcheng

Abstract

γ-amino butyric acid (GABA) is a non-protein amino acid widely distributed in nature. It has analgesic effect,
promotes growth hormone secretion and prevents the physiological function of Alzheimer's disease.In order to
improve the yield of GABA, the strain of strain HX-3-6 was tested by Atmospheric and Room Temperature Plasma
(ARTP), and the strain with more than 90% lethality was selected for fermentation, and through the gradient plate
according to the level of GABA concentration and colony size of the screening. Then, use the fermentation medium
for re-screening and genetic stability test. The yield of the mutant strain L-120-1 was 5.828 g/L, which was 18.84%
higher than that before the mutation (4.904). The mutant strain L-120-1 was used as the starting strain for ARTP
mutagenesis. The yield of the stable mutant strain 90s-high was 6.178g / L, which was 16.15% higher than that before
the mutation (5.319g / L). The yield of HX-3-6 strain was higher than that of GABA mutant strain 90s-high 2, which
was stable and high yield GABA was 25.98%.


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DOI: http://dx.doi.org/10.18063/gse.v1i1.525
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