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The Use of Limited Extension Method in the Detection of Telomerase Activity

Linkai Ji

Abstract

Telomerase is a kind of nuclear protease with special reverse transcriptase activity, which can repair the damage of telomere at the end of chromosome caused by cell division and differentiation. The research shows that telomerase activity is related to cell aging, cell proliferation and a series of related diseases, so the detection method of telomerase activity is of great significance for the study of cell immortalization, aging, cancer and other diseases. This paper introduces the significance of detection of telomerase activity and the limited extension method which has the characteristics of sensitivity, high efficiency, low cost, easy quantification and low operation difficulty.


Keywords

Telomerase; Limited Extension Method; Activity Detection

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References

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DOI: http://dx.doi.org/10.18063/pam.v2i1.1141
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Copyright (c) 2019 Linkai Ji

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