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Physiological and Transcriptional Responses of Acidithiobacillus caldus to Copper Stress

Yaquan Cui, Shoushuai Feng, Hailin Yang

Abstract

High concentrations of copper ions in the leaching environment would affect the normal growth of the leached microorganisms. In this paper, the physiological and transcript levels of Acidithiobacillus caldus under copper stress were analyzed to explore its mechanism of resisting copper stress. The growth of the cells under copper stress was inhibited, and more EPS was produced, more glutamic acid, glycine and cysteine were secreted. The content of unsaturated fatty acids and cyclopropane fatty acids increased, and the level of antioxidants enhanced. 140 genes were significantly differentially expressed under the stress of 1 g/L copper ions while 250 genes under the stress of 3 g/L copper ions. These genes were primarily involved in cellular metabolism, signal transduction, and cell movement. In this paper, the physiological and transcriptional responses of Acidithiobacillus caldus under copper stress were investigated. The results can provide a reference for finding strategies to improve copper resistance.


Keywords

Acidithiobacillus Caldus; Copper Stress; RNA-seq

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References

WATLING H R, WATKIN E L J, RALPH D E, et al. The resilience and versatility of acidophiles that contribute to the bio-assisted extraction of metals from mineral sulphides[J]. Environmental Technology, 2010, 31(8-9): 915-33.

CORDOBA E M, MUNOZ J A, BLAZQUEZ M L, et al. Leaching of chalcopyrite with ferric ion. Part II: Effect of redox potential[J]. Hydrometallurgy, 2008, 93(3): 88-96.

PANDA S, AKCIL A, PRADHAN N, et al. Current scenario of chalcopyrite bioleaching: A review on the recent advances to its heap-leach technology[J]. Bioresource Technology, 2015, 196:694-706.

HAO X, XIE P, ZHU Y G, et al. Copper tolerance mechanisms of Mesorhizobium amorphae and its role in aiding phytostabilization by Robinia pseudoacacia in copper contaminated soil[J]. Environmental Science & Technology, 2015, 49(4): 2328-2340.

OETIKER N, NORAMBUENA R, MART NEZBUSSENIUS C, et al. Possible Role of Envelope Components in the Extreme Copper Resistance of the BiominingAcidithiobacillus ferrooxidans[J]. Genes, 2018, 9(7): 347.

MART NEZBUSSENIUS C, NAVARRO C A, JEREZ C A. Microbial copper resistance: importance in biohydrometallurgy[J]. Microbial Biotechnology, 2017, 10(2): 279-295.

KOH E I, ROBINSON A E, BANDARA N, et al. Copper import in Escherichia coli by the yersiniabactin metallophore system[J]. Nature Chemical Biology, 2017, 13(9): 1016-1021.

MART NEZBUSSENIUS C, NAVARRO C A, ORELLANA L, et al. Global response of Acidithiobacillus ferrooxidans ATCC 53993 to high concentrations of copper: A quantitative proteomics approach[J]. Journal of Proteomics, 2016, 145: 37-45.

Christel S, Herold M, Bellenberg S, et al. Multi-omics Reveals the Lifestyle of the Acidophilic, Mineral-Oxidizing Model Species Leptospirillum ferriphilumT[J]. Applied & Environmental Microbiology, 2018, 84(3).

LEE M, IMLAY J A. The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(20): 8344-8349.

HALLBERG K B, LINDSTR M E B. Characterization of Thiobacillus caldus sp. nov., a moderately thermophilic acidophile [J]. Microbiology, 1994, 140(4): 3451.

MANGOLD S, VALD S J, HOLMES D S, et al. Sulfur Metabolism in the Extreme Acidophile Acidithiobacillus Caldus[J]. Frontiers in Microbiology, 2011, 2(1): 17.

ZHOU Q G, BO F, BO Z H, et al. Isolation of a strain of Acidithiobacillus caldus and its role in bioleaching of chalcopyrite[J]. World Journal of Microbiology & Biotechnology, 2007, 23(9): 1217-1225.

ZHANG L, ZHOU W, LI K, et al. Synergetic effects of Ferroplasma thermophilum in enhancement of copper concentrate bioleaching by Acidithiobacillus caldus and Leptospirillum ferriphilum[J]. Biochemical Engineering Journal, 2015, 93: 142-150.

LIU X, CHEN B, CHEN J, et al. Spatial variation of microbial community structure in the Zijinshan commercial copper heap bioleaching plant[J]. Minerals Engineering, 2016, 94: 76-82.

FOUNTOULAKIS M, LAHM H W. Hydrolysis and amino acid composition analysis of proteins[J]. Journal of Chromatography A, 1998, 826(2): 109.

JUAN Z, GUO-CHENG D, YANPING Z, et al. Glutathione protects Lactobacillus sanfranciscensis against freeze-thawing, freeze-drying, and cold treatment[J]. Applied & Environmental Microbiology, 2010, 76(9): 2989-2996.

ZENG W, QIU G, ZHOU H, et al. Characterization of extracellular polymeric substances extracted during the bioleaching of chalcopyrite concentrate[J]. Hydrometallurgy, 2010, 96(3): 177-180.

GUPTA P, DIWAN B. Bacterial Exopolysaccharide mediated heavy metal removal: A Review on biosynthesis, mechanism and remediation strategies[J]. Biotechnology Reports, 2017, 13: 58-71.

RUNLAN Y U, TAN J X, PENG Y, et al. EPS-contact-leaching mechanism of chalcopyrite concentrates by A. ferrooxidans[J]. Transactions of Nonferrous Metals Society of China, 2008, 18(6): 1427-1432.

DOLGOVA N V, YU C, CVITKOVIC J P, et al. Binding of Copper and Cisplatin to Atox1 is Mediated by Glutathione through the Formation of Metal-Sulfur Clusters[J]. Biochemistry, 2017, 56(24): 3129.

HU Q, WU X, JIANG Y, et al. Differential gene expression and bioinformatics analysis of copper resistance gene afe_1073 in Acidithiobacillus ferrooxidans[J]. Biological trace element research, 2013, 152(1): 91-97.

GUAN N, LI J, SHIN H D, et al. Microbial response to environmental stresses: from fundamental mechanisms to practical applications[J]. Applied Microbiology & Biotechnology, 2017, 101(10): 1-18.

ZHAO Z, SHI H, LIU C, et al. Duckweed diversity decreases heavy metal toxicity by altering the metabolic function of associated microbial communities[J]. Chemosphere, 2018, 203: 76-82.

WANG J, CHEN W, NIAN H, et al. Inhibition of Polyunsaturated Fatty Acids Synthesis Decreases Growth Rate and Membrane Fluidity of Rhodosporidium kratochvilovae at Low Temperature[J]. Lipids, 2017, 52(8): 1-7.

SWAN T M, WATSON K. Membrane fatty acid composition and membrane fluidity as parameters of stress tolerance in yeast[J]. Canadian Journal of Microbiology, 1997, 43(1): 70-77.

WU C, ZHANG J, WANG M, et al. Lactobacillus casei combats acid stress by maintaining cell membrane functionality[J]. Journal of Industrial Microbiology & Biotechnology, 2012, 39(7): 1031-1039.

YUAN Y C, G NZLE M G. Influence of cyclopropane fatty acids on heat, high pressure, acid and oxidative resistance in Escherichia coli[J]. International Journal of Food Microbiology, 2016, 222: 16-22.


DOI: http://dx.doi.org/10.18063/pam.v2i1.1161
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