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Physiological and Transcriptional Responses of Acidithiobacillus caldus to Copper Stress

Yaquan Cui, Shoushuai Feng, Hailin Yang


High concentrations of copper ions in the leaching environment would affect the normal growth of the leached microorganisms. In this paper, the physiological and transcript levels of Acidithiobacillus caldus under copper stress were analyzed to explore its mechanism of resisting copper stress. The growth of the cells under copper stress was inhibited, and more EPS was produced, more glutamic acid, glycine and cysteine were secreted. The content of unsaturated fatty acids and cyclopropane fatty acids increased, and the level of antioxidants enhanced. 140 genes were significantly differentially expressed under the stress of 1 g/L copper ions while 250 genes under the stress of 3 g/L copper ions. These genes were primarily involved in cellular metabolism, signal transduction, and cell movement. In this paper, the physiological and transcriptional responses of Acidithiobacillus caldus under copper stress were investigated. The results can provide a reference for finding strategies to improve copper resistance.


Acidithiobacillus Caldus; Copper Stress; RNA-seq

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