Micronucleus is an abnormal structure in eukaryotic cells. It is the result of various physical and chemical factors,
such as radiation and chemical drugs acting on dividing cells. It is generally believed that micronuclei are originated
in backward chromosomes and fragments that have lost centromere and are formed at the end of mitosis. However,
other experiments suggest that interphase cells can also form micronuclei. In this study, human peripheral blood
lymphocytes were cultured in vitro and cyclophosphamide 20 ug/ml was injected at specifi c times of the cell cycle to
observe the relationship between micronuclei formation and cell cycle. The experimental results confi rmed that the
cell cycle micronucleus formation.

Foot-and-mouth disease is an acute and highly contagious viral infectious disease. Although the foot and mouth
disease vaccine has been applied in some parts of the world since the beginning of the 20th century, the current
epidemic of foot and mouth disease in the world is still serious and constitutes an obstacle to the trade of animal and
animal products in the world. The porcine pseudorabies virus gene deletion strain PRV TK-/ gE-/ LacZ +, which is
constructed by our laboratory, has the advantages of good safety, large capacity and high recombination effi ciency. In
this study, the artifi cial O-type foot-and-mouth disease P1 gene as an antigen gene, UbiP1 fused with ubiquitin (Ub)
as another antigen gene to enhance cellular immunity, and then shRNA designed for FMD 3B gene and porcine IFN-
[gamma] with antiviral and immune-regulatory eff ects. The two functional genes were constructed in turn to construct
a transfer vector with four functional genes. The transfer vector was transfected into TK-/ gE- / LacZ+ cells with PRV
deletion vector, and the plasmids were purifi ed and identifi ed. The recombinant pseudorabies virus, which contains
the six functional genes of P1 gene, UbiP1, shRNA and IFN-γ, was obtained, which laid the foundation for further
construction of new genetically engineered vaccine.

γ-amino butyric acid (GABA) is a non-protein amino acid widely distributed in nature. It has analgesic effect,
promotes growth hormone secretion and prevents the physiological function of Alzheimer's disease.In order to
improve the yield of GABA, the strain of strain HX-3-6 was tested by Atmospheric and Room Temperature Plasma
(ARTP), and the strain with more than 90% lethality was selected for fermentation, and through the gradient plate
according to the level of GABA concentration and colony size of the screening. Then, use the fermentation medium
for re-screening and genetic stability test. The yield of the mutant strain L-120-1 was 5.828 g/L, which was 18.84%
higher than that before the mutation (4.904). The mutant strain L-120-1 was used as the starting strain for ARTP
mutagenesis. The yield of the stable mutant strain 90s-high was 6.178g / L, which was 16.15% higher than that before
the mutation (5.319g / L). The yield of HX-3-6 strain was higher than that of GABA mutant strain 90s-high 2, which
was stable and high yield GABA was 25.98%.

Differentiated somatic cells can be reprogrammed into induced pluripotent stem cells (iPS cells) by introducing
specific transcription factors. This technique avoids immune rejection and ethical problems in stem cell research.
A great revolution in the fi eld of science. As with embryonic stem cells (ES cells), iPS cells are able to self-renew
and maintain undiff erentiated state. In vitro, iPS cells can be induced to diff erentiate into a variety of mature cells,
therefore, iPS cells in theoretical research and clinical applications are extremely valuable. IPS cell diff erentiation
and transplantation in the treatment of blood diseases have a great use, iPS cells can treat nervous system diseases,
to provide in vitro disease model, to study the mechanism of disease formation, screening new drugs and the
development of new to provide a new treatment The The use of iPS cells as a nuclear donor cell, with the appropriate
receptor cells after fusion can be directly obtained transgenic animals. Not only can improve the genetic nature of
animals, but also can break the boundaries of species and get the new animal traits that cannot achieve by using
traditional mating methods. The research of iPS cells has been widely concerned, and it is the research hotspot in cell
biology and molecular biology. In this paper, the defi nition of iPS cells, the acquisition of iPS cells, the history of
development, the signifi cance of research, the progress of research, the application of iPS cells, and the problems of
iPS cells were reviewed.

Pine is a relatively easy genus for intermediate hybridization. It has been widely believed that there should be a
natural hybrid population in the distribution of Pinus massoniona Lamb. and Pinus hangshuanensis Hsia, that is, the
excessive type of external form between Pinus massoniana and Pinus taiwanensis exist. This paper mainly discusses
the traits and clustering analysis of coniferous lozeng in Huangshan scenic area. This study will provide a theoretical
basis for the classification of long and outstanding Huangshan Song and so on. At the same time, it will provide
reference for the phenomenon of gene seepage between the two species.