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Understanding the Habitual Pattern of Concomitant Consumption of Herbs to Alleviate the Symptoms of Ulcerative Colitis by Comparative Proteome Analysis

Songhua Deng, Hairong Long, Duolin Wu, Zhenqiu Xiao, Sai Chen, Chunju He, Zuqing Yang

Article ID: 1295
Vol 7, Issue 1, 2022, Article identifier:1-13

VIEWS - 112 (Abstract) 56 (PDF /) 15 (Supplementary File /) 13 (Supplementary Table 1 /) 16 (Supplementary Table 2 /) 17 (Supplementary Table 3 /) 14 (Supplementary Table 4 /) 17 (Supplementary Table 5)

Abstract

Anchang decoction is an empirical prescription for the treatment of ulcerative colitis in China. In order to better understand its therapeutic function, large efforts have been made to identify its chemical constituents and to unravel the efficacy of its principal constituents. However, the molecular mechanism of its combinations is still unclear. Proteomics application has yielded some positive results in drug development and the identification of potential drug targets, suggesting the potential of this analytical approach to explore the action of molecular mechanisms of herbal formula by robustly addressing dynamic proteome changes. Label-free quantification and parallel reaction monitoring were used to identify differentially expressed proteins in the colon tissue of ulcerative colitis rats, fed with Anchang decoction and mesalazine, respectively. In this study, a total of 1,182 proteins were identified. From GO and KEGG analyses, the proteins of cytoskeleton and cytochrome P450 changed significantly with the occurrence of ulcerative colitis. In the meantime, antigen binding proteins and antioxidant-related proteins turned out to have drastic fluctuations with mesalazine and Anchang decoction. It has also been confirmed that KRT8, MYH11, FLNA, and LMNA are all related to the formation of ulcerative colitis based on parallel reaction monitoring analysis. The increase in FGG in the ulcerative colitis rat model is due to mesalazine, whereas that in KRT8 is due to Anchang decoction. The results from this study provide insights for the mechanism of action of Anchang decoction, which turns out to be an efficient technical pipeline for understanding worldwide medicinal herbs.


Keywords

Traditional Chinese medicine; Ulcerative colitis; Proteomics; Parallel reaction monitoring; Mechanism of action



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References

Hindryckx P, Jairath V, Haens GD, 2016, Acute Severe Ulcerative Colitis: From Pathophysiology to Clinical Management. Nature Reviews Gastroenterology & Hepatology, 13(11): 654.

Khare V, Krnjic A, Frick A, et al., 2019, Mesalamine and Azathioprine Modulate Junctional Complexes and Restore Epithelial Barrier Function in Intestinal Inflammation. Scientific Reports, 9(1): 2842.

Kobayashi T, Siegmund B, Le Berre C, et al., 2020, Ulcerative Colitis. Nature Reviews Disease Primers, 6(1): 74.

Ham M, Moss AC, 2014, Mesalamine in the Treatment and Maintenance of Remission of Ulcerative Colitis. Expert Review of Clinical Pharmacology, 5(2): 113-123.

Chao S-Y, Ye S-J, Wang W-W, et al., 2019, Progress in Active Compounds Effective on Ulcerative Colitis from Chinese Medicines. Chinese Journal of Natural Medicines, 2019(2): 81-102.

Xiao Z, Sun P, 2009, Proceedings of the 13th National Anorectal Academic Exchange Conference of Traditional Chinese Medicine, October 16 to 18, 2009: Clinical Observation and Electron Microscopic Study of Anchang Capsule in the Treatment of Ulcerative Colitis. Shaanxi Traditional Chinese Medicine Publisher, Xi’an, Shaanxi, China, 8.

Xiao Z, He Y, Lv Xi, et al., 1993, Self-Made Anchang Decoction in the Treatment of 50 Cases of Ulcerative Colitis. Guangxi Traditional Chinese Medicine, 1993(03): 3-6.

Sun P, 2017, Intestinal Microecological Changes in Rats with Ulcerative Colitis and the Intervention Mechanism of Anchang Decoction. Guangxi Medical University, Guangxi. https://doi.org/10.7666/d.Y3246157

Liang Y, Sun P, 2021, Effect of Anchang Decoction on Inflammation and Immunity in Rats with Ulcerative Colitis Based on miRNA-146a/JAK/STAT/SOCS-3 Signal Pathway. Chinese Journal of Experimental Traditional Medical Formulae, 2021(24): 30-38.

Liu B, Zheng X, Li J, et al., 2021, Revealing Mechanism of Caulis Sargentodoxae for the Treatment of Ulcerative Colitis Based on Network Pharmacology Approach. Bioscience Reports, 41(1): BSR20204005.

Zhang X, Choi FFK, Zhou Y, et al., 2012, Metabolite Profiling of Plasma and Urine from Rats with TNBS-Induced Acute Colitis Using UPLC-ESI-QTOF-MS-Based Metabonomics – A Pilot Study. Febs Journal, 279(13): 2322-2338.

Wang Y, Chen H, Guo Z, et al., 2017, Quantitative Proteomic Analysis of Iron-Regulated Outer Membrane Proteins in Aeromonas Hydrophila as Potential Vaccine Candidates. Fish Shellfish Immun, 68: 1-9.

Huang DW, Sherman BT, Lempicki RA, 2009, Systematic and Integrative Analysis of Large Gene Lists Using DAVID Bioinformatics Resources. Nature Protocols, 4(1): 44.

Roy SM, Becker CH, 2003, Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling or Spiked Standards. Analytical Chemistry, 75(18): 4818-4826.

MacLean B, Tomazela DM, Shulman N, et al., 2010, Skyline: An Open Source Document Editor for Creating and Analyzing Targeted Proteomics Experiments. Bioinformatics, 26(7): 966-968.

Li Y-H, Sun W, Zhou B-J, 2019, iTRAQ-Based Pharmacoproteomics Reveals Potential Targets of Berberine, A Promising Therapy for Ulcerative Colitis. European Journal of Pharmacology, 850: 167-179.

Pawlak G, Helfman D, 2001, Cytoskeletal Changes in Cell Transformation and Tumorigenesis. Current Opinion in Genetics & Development, 11(1): 41-47.

May D, Pan S, Crispin DA, et al., 2011, Investigating Neoplastic Progression of Ulcerative Colitis with Label-Free Comparative Proteomics. Journal of Proteome Research, 10(1): 200-209.

Liu K, Jia B, Zhou L, et al., 2021, Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics and Lipidomics Identify Biomarkers for Efficacy Evaluation of Mesalazine in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse Model. Journal of Proteome Research, 20(2): 1371-1381.

Montellano P, 2010, Hydrocarbon Hydroxylation by Cytochrome P450 Enzymes. Chemical Reviews, 110(2): 932-48.

Sen A, Stark H, 2019, Role of Cytochrome P450 Polymorphisms and Functions in Development of Ulcerative Colitis. World Journal of Gastroenterology, 25(23): 2846-2862.

Ganapasam S, Pandurangan AK, Kumar S, et al., 2014, Luteolin, a Bioflavonoid Inhibits Azoxymethane-Induced Colon Carcinogenesis: Involvement of iNOS and COX-2. Pharmacognosy Magazine, 10(38): 306-10.

Zhang CL, Zhang S, He WX, et al., 2017, Baicalin May Alleviate Inflammatory Infiltration in Dextran Sodium Sulfate-Induced Chronic Ulcerative Colitis Via Inhibiting IL-33 Expression. Life Sciences, 186: 125-132.

Kim YJ, Ko H, Park JS, et al., 2010, Dimethyl Cardamonin Inhibits Lipopolysaccharide-Induced Inflammatory Factors Through Blocking NF-KappaB P65 Activation. International Immunopharmacology, 10(9): 1127-1134.

Owens DW, Wilson NJ, Hill AJM, et al., 2004, Human Keratin 8 Mutations That Disturb Filament Assembly Observed in Inflammatory Bowel Disease Patients. Journal of Cell Science, 117(Pt 10): 1989-1999.

Pavan E, Damazo AS, Arunachalam K, et al., 2020, Copaifera Malmei Harms Leaves Infusion Attenuates TNBS-Ulcerative Colitis Through Modulation of Cytokines, Oxidative Stress and Mucus in Experimental Rats. Journal of Ethnopharmacology, 267: 113499.

Gleeson MH, Walker JS, Wentzel J, et al., 1972, Human Leucocyte Antigens in Crohn’s Disease and Ulcerative Colitis. Gut, 13(6): 438-440.

Kameyama JI, Narui H, Inui M, et al., 1984, Energy Level in Large Intestinal Mucosa in Patients with Ulcerative Colitis. Tohoku Journal of Experimental Medicine, 143(2): 253.

Bellmann K, 2000, p38-dependent Enhancement of Cytokine-induced Nitric-oxide Synthase Gene Expression by Heat Shock Protein 70. Journal of Biological Chemistry, 275(24): 18172-18179.

Urbanelli L, Emiliani C, Massini C, et al., 2008, Cathepsin D Expression Is Decreased in Alzheimer’s Disease Fibroblasts. Neurobiology of Aging, 29(1): 12-22.

Zarubin T, Han J, 2005, Activation and Signaling of the p38 MAP Kinase Pathway. Cell Research, 15(1): 11-18.

Goldfinger LE, 2008, Choose your Own Path: Specificity in Ras GTPase Signaling. Molecular Biosystems, 2008(4): 293-299.


DOI: http://dx.doi.org/10.18063/jmds.v7i1.1295
(112 Abstract Views, 56 PDF / Downloads15 Supplementary File / Downloads13 Supplementary Table 1 / Downloads16 Supplementary Table 2 / Downloads17 Supplementary Table 3 / Downloads14 Supplementary Table 4 / Downloads17 Supplementary Table 5 Downloads)

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